In-vitro bioaccessibility of antioxidant properties of bee pollen in Turkey

Abstract The study aims to determine certain physicochemical properties (moisture, ash, titratable acidity, pH, and color), total phenolic content, antioxidant capacity (CUPRAC, ABTS, DPPH) and bioaccessibility of 20 bee pollen samples (10 unpackaged and 10 trademarked products in their original packages) sold in Turkey. The total phenolic content of the bee pollen samples was detected as 147.10-462.02 mg GAE/g and bioaccessibility ranged from 36.91% to 77.87%. The highest total value for antioxidant capacity was assessed as 83.24-257.27 μmol TE/g by the CUPRAC method. When the bioaccessibility of the antioxidant capacity of bee pollen was compared among the methods, the highest value (95.76%) was obtained for the sample M by the CUPRAC method. Antioxidant capacity and bioaccessibility of the unpackaged bee pollen samples were lower than those of the trademarked products. Based on overall evaluations, it can be deduced that bee pollen known for its potential beneficial effects on health may well be used as a functional food additive in the production of various food products with its high antioxidant and bioaccessibility features.

摘要 本研究旨在测定土耳其市场售卖的20份蜂花粉样品（10份无包装样品与10份带原包装的品牌产品）的多项理化性质：水分、灰分、可滴定酸度、pH值与色泽，总酚含量、抗氧化能力（CUPRAC、ABTS、DPPH法）以及生物可及性。蜂花粉样品的总酚含量为147.10~462.02 mg GAE/g，生物可及性范围为36.91%~77.87%。采用CUPRAC法测得的抗氧化能力总值最高，为83.24~257.27 μmol TE/g。对比不同检测方法下蜂花粉抗氧化能力的生物可及性时，CUPRAC法测得M样品的抗氧化能力生物可及性达95.76%，为所有样品中最高。无包装蜂花粉样品的抗氧化能力与生物可及性均低于品牌产品。综合评估可知，以潜在健康益处著称的蜂花粉，凭借其优异的抗氧化能力与生物可及性特性，可作为功能性食品添加剂应用于各类食品的生产中。