Core promoterome of barley embryo [CAGE]. Core promoterome of barley embryo [CAGE]

We define the promotoreome sequence and epigenetic architecture in barley embryos to both put it into evolutionary perspective and to serve as a reference for agricultural and research biotechnology in cereals. Overall design: Cap Analysis of Gene Expression conducted on total RNA isolated from three embryonic developmental stages: 8 days after pollination (8DAP), 24 days after pollination (24DAP) and 4 days after germination (4DAG) in two highly correlated replicates本研究解析了大麦胚胎中的启动子组（promotoreome）序列与表观遗传架构，以期从进化视角开展相关研究，并为谷类作物的农业应用与科研生物技术提供参考数据集。整体实验设计：采用基因表达Cap分析（Cap Analysis of Gene Expression, CAGE）技术，对授粉后8天（8DAP）、授粉后24天（24DAP）及萌发后4天（4DAG）三个胚胎发育阶段的总RNA进行分析，实验设置两个高度相关的生物学重复。