mRNA during Spt6,Spt16 and Rtt106 depletion

We measured the transcriptome of yeast cells during the depletion of chromatin regulators using anchor away Overall design: Sensor and anchor-away-system carrying cells were grown to exponential phase and then regualtor depletion was induced using Rapamycin. Sampls for RNA seq were collected ever 10minutes.我们利用锚定远离（anchor away）系统，对染色质调控因子耗竭过程中的酵母细胞转录组进行了检测。 实验设计：将携带传感器及锚定远离系统的酵母细胞培养至指数生长期，随后通过雷帕霉素（Rapamycin）诱导调控因子耗竭，每隔10分钟收集一次用于RNA测序（RNA-seq）的样本。