2009-2010 Outbreak: Salmonella enterica subsp. enterica serovar Typhimurium Genome Sequencing. Salmonella enterica subsp. enterica serovar Typhimurium str. RM14083

To better understand the genomic relationship of Salmonella enterica subsp. enterica serovar Typhimurium isolates sharing the JPXX01.0014 pulse field gel electrophoresis (PFGE) type, we sequenced and analyzed the whole genome shotgun assemblies of five clinical isolates that were isolated across the United States between 2009 and 2010. Three additional isolates possessing the same PFGE type collected from celery in 2002, raw pecans in 2009 and from California pond water in 2009 were also sequenced. Comparison of all of these sequences with the seven completed S. Typhimurium genomes clearly demonstrate that all of these isolates are nearly identical to S. Typhimurium strain 14082, which has been utilized in laboratory experiments for over two decades.

为深入解析共享JPXX01.0014脉冲场凝胶电泳（pulse field gel electrophoresis，PFGE）型别的鼠伤寒沙门氏菌（Salmonella enterica subsp. enterica serovar Typhimurium）分离株之间的基因组关联，我们对2009至2010年间在美国境内分离得到的5株临床分离株开展全基因组测序，并解析其全基因组鸟枪组装（whole genome shotgun assemblies）序列。另有3株携带相同PFGE型别的分离株，分别于2002年从芹菜样本中分离、2009年从生山核桃样本中分离，以及2009年从加利福尼亚州池塘水体中分离，同样完成了全基因组测序。将所有上述序列与7株已完成全基因组组装的鼠伤寒沙门氏菌基因组进行比对分析，结果清晰证实，所有受试分离株均与鼠伤寒沙门氏菌菌株14082高度同源；该菌株已被用于实验室研究超过二十年。