Colorado potato beetle transcriptional response to aegerolysin treatment

Protein complexes of aegerolysins pleurotolysin A2 (PlyA2) and pleurotolysin B (PlyB) from oyster mushrooms Pleurotus sp. display targeted toxicity against Colorado potato beetle (CPB; Leptinotarsa decemlineata) larvae. This selective toxicity is achieved through aegerolysins' interaction with insect-specific membrane sphingolipids. This study explores the potential adaptive response of CPB larvae to the aegerolysin complex. Leptinotarsa decemlineata larvae fed on potato leaves soaked in PlyA2/PlyB/buffer solution vs control larvae fed on leaves soaked in buffer; two sampling time points - 1 and 5 days post treatment

源自侧耳属（Pleurotus sp.）平菇的埃格溶菌素（aegerolysins）家族的侧耳溶菌素A2（PlyA2）与侧耳溶菌素B（PlyB）组成的蛋白复合物，对科罗拉多马铃薯甲虫（CPB；*Leptinotarsa decemlineata*）幼虫具有靶向毒性。该选择性毒性源于埃格溶菌素与昆虫特异性膜鞘脂的相互作用。本研究旨在探究科罗拉多马铃薯甲虫幼虫应对埃格溶菌素复合物的潜在适应性反应。实验设置两组处理：一组为取食浸泡有PlyA2/PlyB/缓冲液溶液的马铃薯叶片的幼虫，另一组为取食仅浸泡缓冲液的马铃薯叶片的对照组幼虫；采样时间点设为处理后1天与5天。