Deep sequencing, profiling and detailed annotation of miRNAs in Takifugu rubripes

We performed extensive transcriptome analysis of the tiger pufferfish (Takifugu rubripes) using the next-generation sequencer SOLiD III, and profiled millions of small RNA sequences in somatic tissues and gonads, as well as identifying thousands of miRNAs. The number of miRNAs identified was the highest in fish species reported to date. We also report highly variable isomiR features. Overall design: miRNA expression profiles of 9 tissues (7-somatic tissues and 2-gonads) of torafugu were generated by deep sequencing using SOLiD3.本研究采用下一代测序仪SOLiD III对虎河豚（Takifugu rubripes）开展了全面的转录组分析，对其体细胞组织与性腺中的数百万条小分子RNA序列进行了表达谱分析，并鉴定出数千个微小RNA（miRNAs）。本研究鉴定得到的miRNAs数量为目前已报道的鱼类物种中最高。此外，我们还报道了高度变异的miRNA异构体（isomiR）特征。整体实验设计：本研究通过SOLiD3深度测序，获取了红鳍东方鲀（虎河豚）9种组织（7种体细胞组织与2种性腺组织）的miRNA表达谱。