Characterization of changes in global genes expression in the distal colon of loperamide-induced constipation SD rats in response to the laxative effects of Liriope platyphylla

In order to characterize the changes in global gene expression in the distal colon of constipated SD rats in response to the laxative effects induced by aqueous extract of Liriope platyphylla (AEtLP) including isoflavone, saponin, oligosaccharide, succinic acid and hydroxyproline, total RNA extracted from the distal colon of AEtLP-treated constipation rats was hybridized to oligonucleotide microarrays.Overall, 581 genes were up-regulated and 216 genes were down-regulated by constipation induced by loperamide, while 67 genes were up-regulated and 421 genes were down-regulated by AEtLP treatment in constipated rats compared to controls. Among the transcripts up-regulated by constipation, 89 were significantly down-regulated and 20 were recovered to normal levels by AEtLP treatment. The major genes in the down-regulated categories included Slc9a5, klk10, Fgf15 and Alpi, while the major genes in the recovered categories were Cyp2b2, Ace, G6pc and Setbp1. However, nine of these genes that were down-regulated by constipation were significantly up-regulated and four were recovered to normal levels by AEtLP treatment. The major genes in the up-regulated categories included Serpina3n, Lcn2 and Slc5a81, while the major genes in the recovered categories were Tmem45a, Rerg and Rgc32. Constipation was induced in SD rats by subcutaneous injection of loperamide for 3 days. At 15 hr after the final treatment of loperamide, each animal were received a consistent volume of water or 15 uL/g body weight of AEtLP (1,000 mg/kg weight) via oral administration for once at 9 AM.

为表征便秘SD大鼠远端结肠的全局基因表达变化，以响应含异黄酮、皂苷、寡糖、琥珀酸与羟脯氨酸的麦冬（Liriope platyphylla）水提物（aqueous extract of Liriope platyphylla，缩写AEtLP）所诱导的润肠作用，我们将经AEtLP处理的便秘大鼠远端结肠提取的总RNA与寡核苷酸微阵列进行杂交。整体而言，洛哌丁胺诱导的便秘模型中，共有581个基因上调、216个基因下调；而与对照组相比，便秘大鼠经AEtLP处理后，有67个基因上调、421个基因下调。在便秘诱导上调的转录本中，有89个经AEtLP处理后显著下调，另有20个恢复至正常水平。下调类别中的核心基因包括Slc9a5、klk10、Fgf15与Alpi；而恢复至正常水平的核心基因为Cyp2b2、Ace、G6pc与Setbp1。然而，在便秘诱导下调的基因中，有9个经AEtLP处理后显著上调，另有4个恢复至正常水平。上调类别中的核心基因包括Serpina3n、Lcn2与Slc5a81；而恢复至正常水平的核心基因为Tmem45a、Rerg与Rgc32。本研究通过皮下注射洛哌丁胺连续3天诱导SD大鼠便秘。末次洛哌丁胺给药15小时后，于上午9点通过灌胃给予各组动物等体积的水或15μL/g体重的AEtLP（剂量为1000mg/kg体重），仅单次给药。