Replacing skeletal muscle alpha-actin with cardiac actin in mouse skeletal muscle. Mus musculus

Skeletal muscle actin mice (Crawford et al., (2002) Mol Cell Biol 22, 5587) were crossed with cardiac actin transgenic mice (termed "ACTC^Coco" or "Coco" for short), to produce mice that had cardiac actin instead of skeletal muscle actin in their skeletal muscles (termed "ACTC^Co/KO" or for short "Coco/KO"). Microarray analysis using the Illumina mouse-6 v1.1 expression beadchip was performed on RNA extraced from the soleus muscle of Coco/KO mice and wildtype mice, to confirm the swith in actin isoform expression, and to determine what other differences might exist between wildtype mice and the Coco/KO mice. Keywords: genetic modification Overall design: 3 RNA samples (each being the pool of two individual samples extracted from different soleus muscles from different individual mice) per genotype (either wildtype or Coco/KO) were used. The total 6 RNA samples were processed using an Illumina mouse-6 v1.1expression beadchip and then the differentially expressed genes determined.

将骨骼肌肌动蛋白小鼠（Crawford等人，2002年，Mol Cell Biol 22卷，5587页）与心脏肌动蛋白转基因小鼠（简称"ACTC^Coco"或"Coco"）杂交，获得在骨骼肌中表达心脏肌动蛋白而非骨骼肌肌动蛋白的工程小鼠，命名为"ACTC^Co/KO"或简称"Coco/KO"。
采用Illumina小鼠-6 v1.1表达微珠芯片（Illumina mouse-6 v1.1 expression beadchip），对Coco/KO小鼠与野生型小鼠的比目鱼肌提取RNA进行微阵列分析，以验证肌动蛋白同工型的表达转换，并探究野生型与Coco/KO小鼠之间存在的其他差异。
关键词：基因修饰
整体实验设计：每种基因型（野生型或Coco/KO）各使用3份RNA样本（每份样本为2只不同个体小鼠的不同比目鱼肌提取样本的混合样品），总计6份RNA样本经Illumina小鼠-6 v1.1表达微珠芯片处理后，完成差异表达基因的鉴定。