MSH1 disruption in plants triggers RdDM-mediated reprogramming of phenotypic plasticity (RNA-Seq). MSH1 disruption in plants triggers RdDM-mediated reprogramming of phenotypic plasticity (RNA-Seq)

To generate epi-lines, two third-generation msh1 T-DNA mutant (SAIL_877_F01) plants were used to pollinate Col-0 to generate two independent F1 populations. Derived F1 progenies were self-pollinated to generate F2 populations that were genotyped for the msh1 T-DNA mutation. MSH1 F2 plants were self-pollinated to produce F3 families that were used in the study. Overall design: 3 plants from each of the epi populations and Col-0 WT control were sequenced.

为构建表观遗传株系（epi-lines），本研究以两株SAIL_877_F01型MSH1基因T-DNA插入第三代突变体植株作为父本，对哥伦比亚野生型（Col-0）进行授粉，以获得两个独立的子一代（F1）群体。将所得F1后代进行自交，得到子二代（F2）群体，并对该群体开展MSH1基因T-DNA插入突变的基因型鉴定。随后，将携带MSH1突变的F2植株进一步自交，获得本研究所用的子三代（F3）家系。实验整体设计：从各表观遗传株系中各选取3株植株，同时设置哥伦比亚野生型（Col-0）对照，对所有供试材料进行测序。