Intermediate filaments are regulators organismal lifespan in C. elegans. Intermediate filaments are regulators organismal lifespan in C. elegans

Purpose: Try to find out whether germline loss retards the normal somatic aging Methods: Employed transcriptome analysis in both young and aged germline-deficient animals and identified genes that are normally decreased during aging in wild-type (WT) worms but are well maintained in aged germline-deficient animals Results: Compared with D1, there were 1834 genes downregulated at D6, while only 441 genes showed increased expression Overall design: Wild type Caenorhabditis elegans and glp-1 mutants are 25°C-cultivated to the D1 adult , and keep at 20°C to D6. Then total mRNA profiles were generated by deep sequencing, in triplicate, using Illumina NovaSeq 6000

研究目的：探究生殖系缺失是否能够延缓正常的体细胞衰老过程。
研究方法：采用转录组分析技术对年轻及衰老的生殖系缺陷动物进行检测，筛选出野生型（Wild-type, WT）秀丽隐杆线虫（Caenorhabditis elegans）在衰老进程中表达量下调，但在衰老的生殖系缺陷动物中仍维持稳定表达水平的基因。
研究结果：与第1天（D1）的样本相比，第6天（D6）的样本中共鉴定出1834个表达下调的基因，仅441个基因呈现表达上调。
整体实验设计：将野生型秀丽隐杆线虫（Caenorhabditis elegans）与glp-1突变体在25℃条件下培养至第1天成虫期，随后转移至20℃继续培养至第6天；随后通过深度测序构建总mRNA转录组图谱，实验设置三次生物学重复，测序平台采用Illumina NovaSeq 6000。