Biomarker discovery for ectoparasite host resistance in cattle

This project aimed to discover the protein-based biomarkers for tick resistance in cattle using cattle skin samples. The cattle were phenotyped into two groups, tick-resistant and susceptible after artificial tick challenge. Mean tick scores were used to categorise cattle. The SWATH analysis was sued to measure the relative abundance of proteins in skin samples of the two groups at different time points.本研究旨在通过牛皮肤样本，挖掘与牛蜱虫抗性相关的蛋白质基生物标志物（protein-based biomarkers）。所有受试牛经人工蜱虫攻毒后，被表型分为蜱虫抗性组与易感组两类，以平均蜱虫寄生评分作为牛只的分组标准。本研究采用SWATH分析技术，对两组牛皮肤样本在不同时间点的蛋白质相对丰度进行定量检测。