Observational study of the dynamic emergence of Ng2-DsRed+ cells before vasculogenesis in a murine embryonic stem cell model of blood vessel development.

Time-lapse images of double-reporter embryonic stem cell (DR-ESC) differentiation over ≈30 h. Magenta arrowheads (vii and ix) denote emergence of endothelial cells (ECs; x and xii). Dashed magenta circles are precursor Flk-1-eGFP-low (enhanced green fluorescent protein) mesoderm (i, iv, and vii; green in iii, vi, and ix). Dashed purple boxes indicated EC coalescence into primitive vessels (x). Cyan arrows are Ng2-DsRed+ cells (ii, v, viii, and xii; red in iii, vi, ix, and xiii) arising before Flk-1-eGFP+ signals. Dashed cyan ovals note Ng2-DsRed+ cell and Flk-1-eGFP+ mesoderm interactions (vi, ix, and xii). Dashed yellow ovals note Ng2-DsRed+ cells interacting with ECs undergoing organization (xii). Time (hh:mm:ss).

本数据集包含双报告基因胚胎干细胞（double-reporter embryonic stem cell, DR-ESC）约30小时分化过程的延时摄影图像。品红色箭头（对应第vii、ix帧）指示内皮细胞（endothelial cells, ECs；对应第x、xii帧）的出现。品红色虚线圆圈标记低表达Flk-1-增强型绿色荧光蛋白（enhanced green fluorescent protein, eGFP）的中胚层前体细胞（对应第i、iv、vii帧，在第iii、vi、ix帧中呈绿色）。紫色虚线框标注内皮细胞汇聚形成原始血管的过程（对应第x帧）。青色箭头指向Ng2-DsRed阳性细胞（对应第ii、v、viii、xii帧，在第iii、vi、ix、xiii帧中呈红色），此类细胞的出现早于Flk-1-eGFP阳性信号。青色虚线椭圆标记Ng2-DsRed阳性细胞与Flk-1-eGFP阳性中胚层的相互作用（对应第vi、ix、xii帧）。黄色虚线椭圆标注Ng2-DsRed阳性细胞与正在发生组织化的内皮细胞之间的相互作用（对应第xii帧）。图像时间标注格式为hh:mm:ss。