IκBζ is a key transcriptional regulator of IL-36-driven psoriasis-related gene expression in keratinocytes

Human primary keratinocytes were lentivirally transfected with control or NFKBIZ shRNA followed by stimulation with 1.5 and 24 h with IL-36. Total RNA was extracted using TRIZOL. After mRNA purification with the NEBNext mRNA Isolation Kit, NEBNext Ultra RNA Library Preparation Kit was used for library construction.将人类原代角质形成细胞以慢病毒转染对照或NFKBIZ短发夹RNA（shRNA），随后用白细胞介素36α（IL-36α）分别刺激1.5小时与24小时。采用TRIZOL试剂提取总RNA，经NEBNext mRNA分离试剂盒（NEBNext mRNA Isolation Kit）纯化mRNA后，使用NEBNext Ultra RNA文库制备试剂盒（NEBNext Ultra RNA Library Preparation Kit）完成文库构建。