Multiorgan Proteomics Reveals Metabolic Synergism of Dietary Chlorella vulgaris and Enterococcus lactis SF68 in Weaning Piglets

Chlorella vulgaris microalgae is an
alternative
feedstuff with an interesting nutritional profile, which may be beneficial
when combined with probiotics. C. vulgaris and Enterococcus lactis SF68 dietary inclusion was studied on
weaning piglet physiology using proteomics. In a 5-week growth trial,
72 piglets were fed a cereal/soybean meal-based diet (Control); Control
plus 5% C. vulgaris (CV5); or CV5 plus probiotic
(CV5P). Proteomics of liver, ileum, and longissimus lumborum was performed by LC-MS/MS. The probiotic effect led to the most
differentially abundant proteins, and C. vulgaris was the lowest by comparison to controls. The combined diet led
to synergistic effects. Liver immunity, lipid mobilization attenuation,
and maintained protein catabolism may foster muscle development. Lower
intestinal ketone oxidation suggests an improved nutritional state,
along with immune priming against bacterial infection. Altered muscle
metabolism (mitochondrial respiration, connective tissue, and lipid
composition) suggests improved meat tenderness and nutritional value.