Bradyrhizobium sp. JGI 0001019-M21. Bradyrhizobium sp. JGI 0001019-M21

Rhizosphere Grand Challenge Single Cell Sequencing. The goal of this project is to comprehensively characterize the genomes of microorganisms found in the rhizosphere and endosphere of Arabidopsis thaliana. Organisms for study have been targeted based on their reproducible association with one or more Arabidopsis genotypes grown in natural soil. To obtain genomes from both culturable and unculturable fractions of the Arabidopsis microbiome, rhizosphere and endosphere samples were disaggregated and individual cells sorted by flow cytometry and amplified by MDA, then microbes of interest were identified by 16S rRNA sequencing and their whole genomes sequenced. Genomic sequences will be analyzed for genes participating in the plant-microbe symbiosis.

根际大挑战单细胞测序（Rhizosphere Grand Challenge Single Cell Sequencing）项目。本项目旨在全面解析拟南芥（Arabidopsis thaliana）根际与内生境中微生物的基因组。本研究的目标微生物均基于其与天然土壤中培养的一种或多种拟南芥基因型的可重复关联进行筛选。为获取拟南芥微生物组中可培养与不可培养组分的基因组信息，研究人员对根际与内生境样本进行解离处理，通过流式细胞术分选单个微生物细胞，并采用多重置换扩增（Multiple Displacement Amplification, MDA）进行扩增；随后通过16S rRNA测序鉴定目标微生物，并对其全基因组完成测序。后续将针对参与植物-微生物共生过程的基因开展基因组序列分析。