Lp(a) and 17K recombinant apo(a) treatment of THP-1 macrophages. Lp(a) and 17K recombinant apo(a) treatment of THP-1 macrophages

Lp(a) and its distinguishing apolipoprotein constituent apo(a) have been shown to elicit proinflammatory responses from monocytes and macrophages. To obtain a global picture of the effect of Lp(a) and apo(a) on the transcriptome of these cells, we treated THP-1 macrophages with 250 nM Lp(a) or apo(a) for 6 hours, harvested RNA from the cells, and then performed RNA-seq using an Illumina NextSeq instrument. Analysis of the data using GO and KEGG strategies revealed a series of proinflammatory genes upregulated by apo(a) and even more dramatically by Lp(a). Overall design: Duplicate wells of THP-1 macrophages were treated for 6 hours with 250 nM Lp(a) or recombinant 17-kringle apo(a), or vehicle alone (phosphate-buffered saline).

脂蛋白(a)（Lipoprotein(a), Lp(a)）及其特异性载脂蛋白组分apo(a)（apolipoprotein(a)）已被证实可诱导单核细胞与巨噬细胞产生促炎应答。为全面解析Lp(a)与apo(a)对该类细胞转录组的整体影响，我们以250 nM浓度的Lp(a)或apo(a)处理THP-1巨噬细胞6小时，提取细胞总RNA后，使用Illumina NextSeq测序仪开展RNA测序（RNA-seq）。采用基因本体论（Gene Ontology, GO）与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）分析策略对数据进行分析，结果显示apo(a)可上调一系列促炎基因，而Lp(a)的上调效果更为显著。实验整体设计：将THP-1巨噬细胞设置复孔，分别以250 nM Lp(a)、重组17-Kringle结构域apo(a)，或仅添加溶剂对照（磷酸盐缓冲液）处理6小时。