Shox2 is necessary for lineage-specific chromatin accessibility in the anterior palate. Shox2 is necessary for lineage-specific chromatin accessibility in the anterior palate

Being a transcription factor, Shox2 binds to specific DNA sites to regulate gene expression via modulating chromatin status. To investigate the impact of Shox2 on the chromatin accessible landscape in the anterior hard palate, we subjected FACS-sorted Shox2+ cells from the palate of E14.5 Shox2Cre/+;Nkx2.5F/F;R26RmTmG and Shox2Cre/-;Nkx2.5F/F;R26RmTmG mice to ATAC-Seq analysis. Overall design: Examination of Shox2 cell linage in Shox2 deficiency palate and the control palate

作为一种转录因子（transcription factor），Shox2可结合特定DNA位点，通过调控染色质状态以调节基因表达。为探究Shox2对前硬腭染色质开放景观的影响，我们将取自E14.5胎龄Shox2Cre/+;Nkx2.5F/F;R26RmTmG与Shox2Cre/-;Nkx2.5F/F;R26RmTmG小鼠腭部、经荧光激活细胞分选（Fluorescence-activated cell sorting, FACS）得到的Shox2阳性细胞，开展了ATAC测序（ATAC-Seq）分析。整体实验设计：分析Shox2缺陷型腭组织与对照腭组织中的Shox2阳性细胞谱系。