Genomic evolution and transmission of Helicobacter pylori. Helicobacter pylori SA172A

The genome sequences of a total of 97 Helicobacter pylori strains were sequenced using 454 pyrosequencing. The sequence data were de novo assembled with the Roche GS De Novo Assembler. Based on these contig assemblies the core genome was determined, which was further used to analyse genome evolution and transmission of H. pylori.本研究采用454焦磷酸测序（454 pyrosequencing）技术，对共计97株幽门螺杆菌（Helicobacter pylori）的基因组序列完成了测序。所获序列数据通过罗氏GS从头组装软件（Roche GS De Novo Assembler）进行从头组装。基于上述重叠群（contig）的组装结果，研究人员确定了幽门螺杆菌的核心基因组，并以此为基础开展该菌的基因组进化与传播机制分析。