Oryza sativa Raw sequence reads. Oryza sativa

We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 in the protospacer of genomes in both protoplasts and regenerated rice, wheat and maize plants我们采用CRISPR-Cas9切口酶-胞嘧啶脱氨酶融合蛋白（CRISPR-Cas9 nickase-cytidine deaminase fusion），在原生质体以及再生水稻、小麦和玉米植株的基因组原间隔序列（protospacer）中，实现了第3至9位胞嘧啶向胸腺嘧啶的靶向转化。