Gene expression in mouse uterus during pre-implantation period after intrauterine administration treatment

Intrauterine peripheral blood mononuclear cells (PBMC) therapy for recurrent implantation failure (RIF) has been reported to improve embryo implantation by acting on the endometrium. However, the exact mode of action of PBMC on the endometrium and the differences in the therapeutic effects of PBMC therapy with and without human chorionic gonadotropin (hCG) are unknown. In this study, we investigated the changes in the endometrium during the implantation phase induced by PBMC administration and the differences in the efficacy of this therapy with and without hCG using a mouse model of implantation failure (IF). IF model was established by the subcutaneous administration of low-dose RU486. Pregnant mice were randomly divided into five groups: control, IF, PBMC, and PBMC-hCG (the latter two groups were IF model mice with intrauterine administration). Uteri from the preimplantation phase (evening of day 3.5) were collected and analyzed using RNA-sequencing (RNA-seq).

已有研究报道，宫腔外周血单个核细胞（peripheral blood mononuclear cells, PBMC）治疗复发性植入失败（recurrent implantation failure, RIF）可通过作用于子宫内膜改善胚胎植入结局。然而，PBMC对子宫内膜的确切作用机制，以及联合与未联合人绒毛膜促性腺激素（human chorionic gonadotropin, hCG）的PBMC治疗方案的疗效差异尚不明确。本研究借助植入失败（implantation failure, IF）小鼠模型，探究PBMC给药后植入窗口期子宫内膜的变化，以及联合与未联合hCG的PBMC治疗方案的疗效差异。本研究通过皮下注射低剂量RU486构建IF模型，将孕鼠随机分为五组：对照组、IF模型组、PBMC治疗组及PBMC-hCG联合治疗组（后两组为经宫腔给药的IF模型小鼠）。于植入前期（妊娠第3.5天傍晚）采集子宫组织，采用RNA测序（RNA-sequencing, RNA-seq）进行分析。