Comparison of RNA editing in single and combined −1 or +1 mutants.

Editing sites are indicated by a C followed by the position of the base on the mature cox2 transcript. Combined mutants present all but C77, C259 and C385 editing sites mutated either at −1 or +1 position in a single construct. Sites C466 and C467 disappeared in the −1 and +1 combined mutants respectively. The numbers indicate the percentage of edited transcripts at the site indicated after sequencing of at least 16 RT-PCR clones.编辑位点以字母C后接成熟cox2转录本（mature cox2 transcript）上对应碱基的位置编号进行标识。组合突变体在单个重组构建体中，对除C77、C259及C385之外的所有编辑位点，在其-1或+1侧翼位置引入突变。C466与C467位点分别在-1和+1组合突变体中消失。文中标注的数值代表对至少16个逆转录聚合酶链式反应（Reverse Transcription Polymerase Chain Reaction, RT-PCR）克隆进行测序后，对应编辑位点的转录本编辑百分比。