CUT&TAG analysis of H3K27ac in granulosa cells from control and POI patients

To reveal the role of NCOA7 in cellular senescence, we performed the CUT&TAG assay using the H3K27ac antibody to map acetylation in granulosa cells from control and POI patients. We conducted the DNA sequencing of libraries from CUT&TAG assay using the H3K27ac antibody in human primary granulosa cells. Overall design: CUT&TAG-seq for acetylation using the H3K27ac antibody in granulosa cells of control and POI patients.为阐明核受体辅激活蛋白7（NCOA7）在细胞衰老中的功能，我们采用组蛋白H3赖氨酸27乙酰化（H3K27ac）抗体开展CUT&TAG实验，对对照组与原发性卵巢功能不全（Primary Ovarian Insufficiency, POI）患者的颗粒细胞内的乙酰化修饰进行图谱绘制。随后，我们针对人类原代颗粒细胞中经H3K27ac抗体介导的CUT&TAG实验所构建的文库完成了DNA测序。整体实验设计：针对对照组及POI患者的颗粒细胞，使用H3K27ac抗体开展CUT&TAG测序以检测乙酰化修饰。