H3K4me2 Chromatin Immunoprecipitation sequencing analysis of chicken Embryonic stem cells, Primordial germ cells and Spermatogonial stem cells

We report the chromatin status in chicken Embryonic stem cells（ESCs）, Primordial germ cells (PGCs) and Spermatogonial stem cells (SSCs). H3K4me2 antibody was used to collect DNA sequences through CHIP experiments, and the DNA was analyzed by next-generation sequencing. We mapped the genome-wide chromatin maps of chicken ESCs, PGCs, and SSCs. We found that the entire genome of H3K4me2 in ESCs, PGCs, and SSCs was erased and then reconstructed. Most of the enriched genes were related to the cell lineage. It is mainly enriched in distal intergenic. H3K4me2 in the promoter first enriches in ESCs, then decreases in PGCs. The enrichment rebuilds in SSCs. This study provides a basis for analyzing the differences of chromatin status in ESCs, PGCs, and SSCs. Examination of H3K4me2 modifications in 3 cell types.

我们报道了鸡胚胎干细胞（Embryonic Stem Cells, ESCs）、原始生殖细胞（Primordial Germ Cells, PGCs）与精原干细胞（Spermatogonial Stem Cells, SSCs）的染色质状态。本研究使用H3K4me2抗体，通过染色质免疫沉淀（Chromatin Immunoprecipitation, ChIP）实验富集得到DNA序列，并借助下一代测序（Next-Generation Sequencing, NGS）完成序列分析，成功绘制了鸡ESCs、PGCs与SSCs的全基因组染色质图谱。研究发现，三类细胞中H3K4me2修饰的全基因组区域均经历了先清除后重建的动态过程。绝大多数富集基因与细胞谱系密切相关，且该修饰主要富集于基因间远端区域。启动子区域的H3K4me2富集水平首先在ESCs中达到峰值，随后在PGCs中逐渐降低，并在SSCs中重新恢复富集模式。本研究为解析ESCs、PGCs与SSCs之间的染色质状态差异提供了重要理论依据，同时完成了三类细胞的H3K4me2修饰检测分析。