scRNA-seq analysis of the open and closed sides of the mouse olfactory epithelium following unilateral naris occlusion

The objectives of this study were to characterize the effects of olfactory deprivation via unilateral naris occlusion (UNO) on the relative abundance of distinct cell types and subtypes. Overall design: Profiling the transcriptomes of single cells dissociated from the open and closed sides of the olfactory epithelia of PD28 mice following unilateral naris occlusion at PD14

本研究旨在阐明通过单侧鼻孔阻塞（unilateral naris occlusion, UNO）实现的嗅觉剥夺对不同细胞类型及亚型相对丰度的影响。实验整体设计如下：于PD14时期对小鼠实施单侧鼻孔阻塞，待其发育至PD28天时，分别从其嗅觉上皮的阻塞侧与开放侧解离单细胞，并对这些单细胞的转录组进行分析。