Amplicon sequencing of Tara Oceans RNA samples corresponding to size fractions for protists.. ARH

Analysis of RNA tags in Tara Oceans Protists size fractions through amplicon sequencing: Seawater was filtered from different depths to retain small and large cell sizes. The RNA was extracted, cDNA prepared then amplified by PCR.基于扩增子测序的塔拉海洋（Tara Oceans）原生生物粒径分级样本RNA标签分析：研究人员从不同深度采集海水并过滤，以留存小型与大型细胞组分；随后提取总RNA，制备互补脱氧核糖核酸（cDNA），再通过聚合酶链式反应（PCR）完成扩增。