miRNAs involed in lactation of cow mammary gland. Bos taurus

We investigated miRNA expression in Holstein dairy cow of mammary gland with different producing quality milk using high-throughput sequence and qRT-PCR techniques. miRNA libraries were constructed from mammary gland tissues taken from a high producing quality milk and a low producing quality milk Holstein dairy cow, the small RNA digitalization analysis based on HiSeq high-throughput sequencing takes the SBS-sequencing by synthesis.The libraries included 4732 miRNAs. A total of 124 miRNAs in the high producing quality milk mammary gland showed significant differences in expression compared to low producing quality milk mammary gland (P<0.05). Conclusion: Our study provides a broad view of the bovine mammary gland small RNA expression profile characteristics. Differences in types and expression levels of miRNAs were observed between high producing quality milk and a low producing quality milk Holstein dairy cow Overall design: Examination of 3 group of different producing quality milk mammary gland of Holstein dairy cow,each group has 3 sample本研究采用高通量测序与实时荧光定量PCR（qRT-PCR）技术，分析泌乳品质不同的荷斯坦奶牛乳腺组织中的微小RNA（miRNA）表达水平。研究人员从泌乳品质高低不同的荷斯坦奶牛乳腺组织中构建miRNA文库；基于HiSeq高通量测序的小RNA数字化分析采用边合成边测序（Sequencing by Synthesis，SBS）技术。本次构建的文库共涵盖4732个miRNA。与泌乳品质较低的荷斯坦奶牛乳腺组织相比，泌乳品质较高的荷斯坦奶牛乳腺组织中共有124个miRNA的表达量存在显著差异（P<0.05）。结论：本研究全面解析了牛乳腺组织小RNA的表达谱特征。研究发现，泌乳品质不同的荷斯坦奶牛之间，其乳腺组织内miRNA的种类与表达水平均存在差异。总体实验设计：选取3组泌乳品质不同的荷斯坦奶牛乳腺组织进行检测，每组包含3个样本。