The study of SWI/SNF subunits and other transcription factors deposition in asynchronous and mitotic mESCs and cells released from mitosis II

We conduct ATAC-seq in SMARCE1 MD/MD(R42A) after mitotic release 90 min in either DMSO treated or BRG1 inhibitor BRM014 treated conditions Examination of chromatin accessibility in mitotically degraded SMARCE1 and control on either DMSO or BRG1 inhibitor BRM014 treated ES cells.

我们在经二甲基亚砜（DMSO）处理或BRG1抑制剂BRM014处理的两种条件下，对有丝分裂释放90分钟后的SMARCE1 MD/MD(R42A)胚胎干细胞开展转座酶可及性染色质测序（ATAC-seq）实验。本研究旨在检测经上述两种处理的胚胎干细胞中，有丝分裂降解的SMARCE1及其对照细胞的染色质可及性。