Bt Cry toxin resistance mapping in Helicoverpa zea F2 crosses

The goal of this study was to use genetic crosses between Helicoverpa zea Cry toxin resistant and susceptible parents to identify genomic regions associated with Cry resistance. Growth of F2 offspring was measured on diets containing Cry1Ab and Cry1A.105 + Cry2Ab2 expressing leaf tissue and control diets containing non-Bt expressing leaf tissue. We used ddRAD sequencing of parents and offspring from these genetic crosses to generate markers across the genome for association with growth phenotype.