T cell response to CD200+/- acute myeloid leukemia

Purpose: Understanding immunosuppression in AML is critical . The goals of this study are to compare the transcriptomics of human CD3+ T cells from humanized mice exposed to CD200+/- AML. Methods: NSG mice were humanized with 10x10^6 healthy PBMCs prior to the introduction of CD200+ or CD200- OCI-AML3 cells. After 10 days, CD3+ T cells were isolated and RNA was sequenced. Results: T cells from mice with CD200+ AML were characterized by an enrichment of T cell activation pathways and a downregulation of numerous metabolic pathways, including OXPHOS and glycolysis. Conclusions: Our results demonstrate, for the first time, that CD200+ AML can directly impair T cell metbolism. Overall design: RNA-seq analysis of sorted CD3+ human T cells extracted from PBMC-humanized mice with either CD200+ (n=3), CD200- (n=3) OCI-AML3 leukemia, or controls (n=2). Results provide insight into the mechanisms of CD200 immunosuppression.