The transcriptome of the parapoxvirus Orf virus reveals novel promoters for heterologous gene expression by poxvirus vectors

RNA-sequencing (RNA-Seq) was used to analyze the transcriptome of parapoxvirus orf virus (ORFV) during infection in primary ovine turbinate cells. Based on the transcriptional profile of individual ORFV genes, we identified ORFV promoters with strong early and late activity and have shown that they can be used to express heterologous genes in ORFV vectors.

本研究采用RNA测序（RNA-sequencing, RNA-Seq），对原代绵羊鼻甲细胞感染副痘病毒羊口疮病毒（ORFV）过程中的病毒转录组展开分析。基于单个ORFV基因的转录谱，我们鉴定出具备强早期及晚期转录活性的ORFV启动子，并证实此类启动子可用于在ORFV载体中表达外源基因。