Bulk and Size-Fractionated Chlorophyll and Phaeopigment Concentrations Collected by Niskin Bottle, BC, Canada (Provisional)

This dataset is comprised of Hakai Institute chlorophyll and phaeopigment concentrations collected from 2014 to present at stations within the Calvert Island, Johnstone Strait, and the Quadra Island study regions. Samples for these data were collected in the field using Niskin bottles. Sample water (250 ml) was filtered through: 1) a single glass fiber filter (GF/F, nominal pore size 0.7 um) to comprise a bulk measure of phytoplankton chlorophyll and phaeopigment concentrations and 2) a stack of filters (GF/F, 3 um, and 20 um polycarbonate filters) to estimate chlorophyll and phaeopigment concentrations from pico, nano, and micro-phytoplankton, respectively. Filters were extracted for 24 hours in 10 ml of 90% acetone and analyzed on Turner Designs Trilogy laboratory fluorometers using the acidification module and following the method of Holm Hansen et al. (1965).

Data have been quality controlled by manual inspection which included a comparison of bulk concentrations against the sum of the size-fractionated filter concentrations. Where available, further comparisons were done with other measures of chlorophyll concentrations including in situ CTD chlorophyll fluorescence and high-performance liquid chromatography (HPLC) data.

Data were collected by the Hakai Institute Oceanography and Nearshore programs.

本数据集涵盖了Hakai研究所（Hakai Institute）于2014年至今，在卡尔弗特岛（Calvert Island）、约翰斯通海峡（Johnstone Strait）与夸德拉岛（Quadra Island）研究区域内各站点采集的叶绿素与脱镁色素（phaeopigment）浓度数据。
该数据集的样本均采用尼斯金采水器（Niskin bottle）在野外现场采集。采集得到的250毫升水样将通过两种方式过滤：其一，使用单张玻璃纤维滤膜（GF/F，标称孔径0.7微米），用以获取浮游植物总叶绿素与脱镁色素的总量测定浓度；其二，使用叠层滤膜组（包含GF/F滤膜、3微米孔径聚碳酸酯滤膜与20微米孔径聚碳酸酯滤膜），分别用以估算超微型浮游植物（pico-phytoplankton）、微型浮游植物（nano-phytoplankton）与小型浮游植物（micro-phytoplankton）的叶绿素与脱镁色素浓度。将滤膜置于10毫升90%丙酮（acetone）溶液中萃取24小时，随后使用酸化模块（acidification module），参照Holm-Hansen等人（1965）的实验方法，在Turner Designs Trilogy实验室荧光计上完成样品分析。
本数据集已通过人工检视开展质量控制，具体包括将总量测定浓度与粒径分级滤膜的浓度总和进行比对。在条件允许的情况下，还会与其他叶绿素浓度测量结果进行进一步比对，包括原位CTD叶绿素荧光数据与高效液相色谱（HPLC）数据。
本数据集由Hakai研究所海洋学与近岸项目组采集完成。