Single-cell multimodal sequencing data from: Single-cell genomics details the maturation block in BCP-ALL and identifies therapeutic vulnerabilities in DUX4-r cases

This dataset contains single-cell multimodal data (scRNA-, scBCR-, and scADT-seq) for twenty-three cases of childhood BCP-ALL, including four samples each of BCR::ABL1-positive, ETV6::RUNX1-positive, and HeH, together with eleven samples of DUX4-rearranged ALL. In addition, the datset contains 9 samples of mononuclear cells, sorted CD34+ (n=3), CD19+ (n=3), and unsorted (n=3), from five normal bone marrow from healthy donors. The dataset is available as raw sequencing reads (fastq; restricted access) or as an annotated multimodal dataset (h5ad). Cells from bone marrow were used for single-cell library preparation, according to the manufacturer’s protocol (10X Genomics CG000208: Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1). The libaries were sequenced on a Novaseq 6000.

本数据集包含23例儿童B细胞前体急性淋巴细胞白血病（B-cell precursor acute lymphoblastic leukemia, BCP-ALL）的单细胞多组学数据，涵盖单细胞RNA测序（scRNA-seq）、单细胞BCR测序（scBCR-seq）及单细胞ADT测序（scADT-seq）。其中BCR::ABL1阳性、ETV6::RUNX1阳性及HeH亚型各有4例样本，另包含11例DUX4重排型急性淋巴细胞白血病样本。
此外，数据集还纳入来自5名健康供者正常骨髓的9份单个核细胞样本，包括分选的CD34+（n=3）、CD19+（n=3）以及未分选样本（n=3）。
该数据集可通过原始测序读数（fastq格式；受限访问）或注释型单细胞多组学数据集（h5ad格式）获取。
所有骨髓来源细胞均按照制造商提供的实验方案（10X Genomics CG000208：Chromium Next GEM单细胞V(D)J试剂试剂盒v1.1）进行单细胞文库制备，随后将文库在NovaSeq 6000测序平台上完成测序。