Additional file 9 of The transcriptome profile of human trisomy 21 blood cells

Additional file 9: Supplementary Table 8. Real-Time RT-PCR experiments performed on 6 subjects with Down syndrome (A5; A6; A7; A8; A9; A10) and 6 normal controls (B5; B6; B7; B8; B9; B10). The tables on the left report the mean of cycles (Cq mean) for the five genes selected and for the reference gene (GAPDH), the difference of Cq means among the gene and GAPDH gene (∆Cq) and finally the relative expression values of the gene vs GAPDH gene obtained through the 2-∆Ct method (2-∆Ct) for each subject. The presence of strong outliers in the expression values of each gene is reported in red in tables on the left. The following is reported in the tables on the right: mean gene expression values (Mean); standard deviation (S.D.); and ratio between DS and N mean gene expression values (Ratio DS/N) after the exclusion of strong outliers.

附加文件9：补充表8。本研究针对6名唐氏综合征（Down syndrome）受试者（编号A5、A6、A7、A8、A9、A10）及6名正常对照个体（编号B5、B6、B7、B8、B9、B10）开展了实时荧光定量反转录聚合酶链反应（Real-Time RT-PCR）实验。左侧表格展示了5个目标基因及内参基因GAPDH的循环阈值均值（Cq mean）、目标基因与GAPDH的Cq均值差值（∆Cq），以及针对每个受试者通过2^(-∆Ct)法（2-∆Ct）计算得到的目标基因相对于GAPDH的相对表达量；左侧表格中以红色字体标注出各基因表达量中的极端异常值。右侧表格则展示了剔除极端异常值后的各项数据：基因平均表达量（Mean）、标准差（S.D.），以及唐氏综合征组与正常对照组的基因平均表达量比值（Ratio DS/N）。