Comprehensive DNA methylation analysis of neuronal and non-neuronal nuclei in the human brain. Homo sapiens

We performed comprehensive DNA methylation analysis in neuronal and non-neuronal nuclei obtained from the human prefrontal cortex. This revealed that neuronal and non-neuronal nuclei manifest qualitatively and quantitatively distinctive DNA methylation patterns. Overall design: Profiling methylation pattern using methylated DNA enriched fraction by MethylCollector vs unmethylated DNA enriched fraction by McrBC treatment. Bar files (see Sample supplementary files) were generated by MAT software and contain MAT scores in each probe. Three bed files (see supplementary files linked below) contain high quality methylated regions in the neuronal, non-neuronal, or both fractions. After the consideration of effect of restriction enzymes, they are extracted based on the commonly methylated regions in all the relevant samples and that MAT score p values < 0.0001 in each sample.

本研究针对从人类前额叶皮层分离获得的神经元与非神经元细胞核，开展了全面的DNA甲基化（DNA methylation）分析。结果显示，神经元与非神经元细胞核在DNA甲基化模式上兼具定性与定量层面的显著差异。实验整体设计：分别采用MethylCollector富集的甲基化DNA组分，与经McrBC酶处理富集的未甲基化DNA组分，对甲基化模式进行分型分析。条形图文件（详见样本补充材料）由MAT软件生成，包含每个探针（probe）的MAT评分。三份BED文件（详见下方链接的补充材料）分别包含神经元组分、非神经元组分，或两组分共有的高质量甲基化区域。在考量限制性内切酶的影响后，上述区域的筛选标准为：覆盖所有相关样本的共有甲基化区域，且每个样本中对应MAT评分的P值均小于0.0001。