Characterization of bacterial pathogens from buffalo mastitis in Bangladesh

This project provides a comprehensive characterization of bacterial pathogens isolated from buffalo milk in Bangladesh, sourced from animals with both clinical and subclinical mastitis. We employed a multi-faceted approach, starting with phenotypic analysis including culture, biochemical tests, and antibiograms. For precise identification, molecular techniques such as PCR and 16S rRNA gene sequencing were utilized. Finally, whole-genome sequencing of key isolates offers deep genomic insights into their virulence factors and antimicrobial resistance genes. This dataset is vital for developing effective control strategies and monitoring antibiotic resistance in the region's dairy industry.本项目对孟加拉国水牛乳汁中分离得到的病原菌进行了全面表征，这些菌株均分离自患有临床型和亚临床型乳腺炎的水牛。本研究采用多维度研究策略，首先开展表型分析，涵盖细菌培养、生化检测以及药敏谱（antibiograms）。为实现精准菌种鉴定，本研究采用了聚合酶链式反应（PCR）与16S rRNA基因测序（16S rRNA gene sequencing）等分子生物学技术。最后，针对关键分离株开展全基因组测序，可深入解析其携带的毒力因子与抗菌药物耐药基因。本数据集对于制定该地区乳制品行业的有效防控策略以及监测抗菌药物耐药性具有重要价值。