Streptococcus mutans transcriptome in the presence of sodium fluoride and sucrose

Considering the diverse mechanisms by which fluoride could affect oral bacteria, this study evaluated the effect of sodium fluoride on Streptococcus mutans UA159 transcriptome in the presence of sucrose, using a high-throughput mRNA-sequencing.S. mutans UA159 was cultured in 3 different types of media: medium control group [Tryptone-yeast], sucrose control group [Tryptone-yeast + 10mM sucrose], and sodium fluoride sucrose test group [Tryptone-yeast + 10mM sucrose + 100ppm NaF].鉴于氟化物可通过多种机制影响口腔细菌，本研究采用高通量mRNA测序（mRNA-sequencing）技术，评估了蔗糖存在条件下氟化钠对变形链球菌（Streptococcus mutans）UA159转录组的影响。变形链球菌UA159分别于三种不同培养基中培养：基础培养基对照组[胰蛋白胨-酵母提取物培养基（Tryptone-yeast）]、蔗糖对照组[胰蛋白胨-酵母提取物培养基+10mM蔗糖]以及氟化钠蔗糖实验组[胰蛋白胨-酵母提取物培养基+10mM蔗糖+100ppm氟化钠（NaF）]。