Cryo-electron microscopy EPN BAG

At the EPN campus level structural biology work is carried out in all the 4 institutes (IBS, ESRF, ILL and EMBL). Today all structural biology groups are turning to cryo-EM. The EM demand is therefore extremely high and present in the 4 Institutes. In a nutshell, there are more than 45 PIs that could benefit from Krios time on the EPN campus (more than 90 different samples). Around 20 % of the sample are ready for high resolution data collection with already prepared grids that have been pre-screened on Glacios microscopes. Whereas some work could be carried out on Glacios microscopes, for difficult samples the use of a krios is indispensable (heterogeneous sample or limited concentration requiring a large number of images; small protein etc). Only these samples will be selected for Krios time at the BAG level

EPN园区内的4家研究机构（IBS、ESRF、ILL与EMBL）均开展结构生物学研究工作。当前，所有结构生物学研究团队均已转向冷冻电子显微镜（cryo-Electron Microscopy，cryo-EM）技术，因此4家机构对冷冻电镜的需求极为旺盛且覆盖所有院所。简言之，园区内超过45名首席研究员（Principal Investigators，PIs）可通过使用Krios冷冻电镜系统的机时获益，相关样本共计90余种。其中约20%的样本已完成制备，可直接开展高分辨率数据采集，且其载网已通过Glacios冷冻电镜完成预筛查。尽管部分实验可在Glacios冷冻电镜上完成，但针对复杂样本（如异质性样品、浓度有限需大量成像的样品、小型蛋白样品等），使用Krios冷冻电镜系统是不可或缺的。仅此类样本将被纳入光束线应用组（BAG）层面的Krios机时分配遴选范围。