Effect of curcuminoids on DPPH radical scavenging

Scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals by 3b, cysteine, and ascorbic acid (2–200 µM).
Raw data including the absolute values (absorbance subtract ethanol blank) and normalized data (% of control) of all individual experiments was uploaded.
The methods and results were published in Rao et al., Biochem Pharmacol. 2022 Sep:203:115202. doi: 10.1016/j.bcp.2022.115202 
Vehicle (ethanol), compound 3b, cysteine hydrochloride (reference compound, Merck) or ascorbic acid (reference compound, Caesar & Loretz GmbH, Hilden, Germany) were incubated with 2,2-diphenyl-1-picrylhydrazyl (Merck; 50 µM) under shaking in the dark. After 30 min, the absorbance was measured at 520 nm using a Multiskan Spectrum Microplate Reader (Thermo Fisher Scientific).

本数据集涵盖3b、半胱氨酸（cysteine）与抗坏血酸（ascorbic acid）对2,2-二苯基-1-苦基肼基（2,2-diphenyl-1-picrylhydrazyl，DPPH）自由基的清除活性实验，受试化合物浓度区间为2–200 μM。
本次上传了全部独立实验的原始数据，包含吸光度扣除乙醇空白后的绝对吸光度值，以及归一化的对照组百分比（% of control）数据。
本研究的方法与结果已发表于Rao等学者2022年9月刊载于《Biochem Pharmacol》的论文，卷203，文章编号115202，DOI：10.1016/j.bcp.2022.115202。
实验流程为：以溶剂（乙醇）、化合物3b、盐酸半胱氨酸（参比化合物，默克（Merck）公司生产）或抗坏血酸（参比化合物，德国希尔登市Caesar & Loretz GmbH公司生产），与2,2-二苯基-1-苦基肼基（DPPH，默克公司生产，浓度50 μM）在避光条件下振荡孵育。
孵育30分钟后，使用Multiskan Spectrum多功能酶标仪（Thermo Fisher Scientific）在520 nm波长下测定吸光度。