Differential gene analysis of extraction sockets in periodontitis in mice

In order to determine the mechanism of the effect of periodontitis on the healing of tooth extraction sockets in mice, the mandibular first molar extraction models of mice in the periodontitis group and the control group were constructed respectively, and samples were taken for RNA-Seq at three time points: 0d, 3d, and 7d. Analysis of key genes in periodontitis regulating tooth extraction socket healing was conducted. Overall design: The mandibular first molar extraction models of mice in the periodontitis group and the control group were constructed respectively, and samples were taken for RNA-Seq at three time points: 0d, 3d, and 7d, with three biological replicates in each group. 18 samples in total.为明确牙周炎对小鼠拔牙创愈合的作用机制，本研究分别构建牙周炎组与对照组小鼠的下颌第一磨牙拔牙模型，并于0d、3d、7d三个时间点采集样本进行RNA测序（RNA-Seq），以此分析牙周炎调控拔牙创愈合的关键基因。整体设计：本研究分别构建牙周炎组与对照组小鼠的下颌第一磨牙拔牙模型，在0d、3d、7d三个时间点采样进行RNA-Seq，每组设置3次生物学重复，总计18个样本。