An PWI- and RRM Motif-Containing Protein Is Critical for Pre-mRNA Splicing and ABA Responses in Arabidopsis. Arabidopsis thaliana

We used RNA-seq platform to determine role of a splicing factor RBM25 in regulation of gene expression and pre-mRNA splicing. We found that a loss-of-function allele of RBM25, rbm25-1, causes up- and down-regulation of a large number of genes. We further found that the rbm25-1 mutation results in defects in altenative splicing of transcripts of many genes including signal transduction components in stress responses. Overall design: Examination of mRNA levels in bulked individual wild type and rbm25-1 mutant seedlings before and after ABA treatment.

本研究借助RNA测序（RNA-seq）平台，解析剪接因子RBM25在基因表达调控与前体mRNA（pre-mRNA）剪接过程中的功能。研究发现，RBM25的功能缺失等位基因rbm25-1可导致大量基因出现表达上调与下调的变化。本研究进一步发现，rbm25-1突变会引发众多基因转录本的可变剪接缺陷，其中涵盖胁迫响应通路中的信号转导相关组分。
实验整体设计：检测混合收集的野生型与rbm25-1突变体幼苗在脱落酸（ABA）处理前后的mRNA表达水平。