Human induced pluripotent stem cells (iPSCs) derived from T-cell compared with that from adult dermal fibroblast (aHDF) [gene expression]. Homo sapiens

In this study we determine the transcriptional profile by microarray of iPSCs and iPSC-derived neurospheres generated from T-cells or aHDF by using direct neurosphere method. Overall design: iPSCs and iPSC-derived tertiary neurospheres generated from T-cells or aHDF subjected to the gene expression microarray analysis.

本研究采用直接神经球诱导法（direct neurosphere method），通过基因芯片（microarray）技术对由T细胞或成人真皮成纤维细胞（adult human dermal fibroblasts, aHDF）重编程获得的诱导多能干细胞（induced pluripotent stem cells, iPSCs），以及该诱导多能干细胞分化得到的神经球（neurospheres）开展转录谱（transcriptional profile）分析。整体实验设计：针对由T细胞或成人真皮成纤维细胞制备的诱导多能干细胞，以及该诱导多能干细胞分化得到的第三代神经球（tertiary neurospheres）开展基因表达芯片分析。