hepatitis A virus Raw sequence reads- Nucleotide variations of Hepatitis A virus in culture and clinical samples detected by NGS

In this study, we developed and validated with pyrosequencing, a model of SNP detection on Hepatitis A virus with NGS. Point mutations in serially passage HAV HM175 clone 1 strain cultured in FRhK4 cells and a wide type HM175 positive stool sample were investigated. This project will be helpful for identifying potential multiple viruses (quasispecies) contamination on a single food item.

本研究采用焦磷酸测序（pyrosequencing）技术，开发并验证了一套基于下一代测序（Next-Generation Sequencing, NGS）的甲型肝炎病毒（Hepatitis A virus, HAV）单核苷酸多态性（Single Nucleotide Polymorphism, SNP）检测模型。本研究针对在FRhK4细胞中连续传代培养的HAV HM175克隆1毒株，以及野生型HM175阳性粪便样本中的点突变展开了分析。该项目可用于识别单一食品样本中潜在的多病毒（准种，quasispecies）污染情况。