Time series ATAC-seq of Th1 differentiation: The effect of Progesterone on Th1 differentiation?. Time series ATAC-seq of Th1 differentiation: The effect of Progesterone on Th1 differentiation?

T-helper 1 responses are involved in the development of many autoimmune diseases such as Multiple sclerosis (MS). MS is a relapse remitting disease that eventually progresses to a progressive neurodegenerative disease. During pregnancy the relapse rate of MS is significantly reduced with peak reduction during the third trimester followed by an increase in relapse rate post-partum. One of the highest expressed hormones during pregnancy is progesterone. Progesterone has been show to have an inhibiting effect on T-cell activation and been proposed to promote a T-regulatory like cell state. To evaluate the influence of progesterone on the chromatin and gene expressive state of T-helper type 1 cells we differentiated primary human naïve T-cell in the presence progesterone with sampling at 0.5, 1, 2, 6 and 24 hours and performed ATAC-seq and RNA-seq.

辅助性T细胞1（T-helper 1）应答参与多发性硬化（Multiple sclerosis, MS）等多种自身免疫病的发生发展。多发性硬化是一种复发缓解型疾病，最终可进展为进行性神经退行性疾病。妊娠期间多发性硬化的复发率显著降低，且于妊娠晚期（third trimester）达到复发率降低的峰值，随后产后复发率再度升高。妊娠期间表达水平最高的激素之一为孕酮（progesterone）。已有研究表明孕酮可抑制T细胞活化，并被提议促进类调节性T细胞状态。为评估孕酮对辅助性T细胞1的染色质及基因表达状态的影响，本研究在孕酮存在的条件下对原代人初始T细胞（primary human naïve T-cell）进行分化，并于0.5小时、1小时、2小时、6小时及24小时时采集样本，随后开展了转座酶可及性测序（ATAC-seq）及RNA测序（RNA-seq）实验。