Pathological scoring.

ObjectiveTo evaluate liver function using dynamic hepatobiliary single-photon emission computed tomography (SPECT) in different rat models of acute liver failure.MethodsTwenty-four 6–8-week-old male Sprague–Dawley rats (weight 190–200 g) were evenly divided into four groups. Acute liver failure was induced by intraperitoneal injection of D-galactosamine (D-GalN, 600 mg/kg) and lipopolysaccharide (LPS, 10 µg/kg), common bile duct ligation surgery, and removing 70% of the liver mass. The fourth group served as the control without intervention. The time-activity curves for the liver and heart were generated from dynamic SPECT scans with 99mTc-ethylene hepatobiliary iminodiacetic acid (EHIDA). Image-derived functional parameters (5-minute heart/liver index [HLI5] and 15-minute receptor index [LHL15]) were calculated. Furthermore, correlations of image-derived parameters with serum interleukin-6 (IL-6) levels, liver aspartate aminotransferase (AST) and alanine transaminase (ALT) levels, and liver mRNA expression levels of tumor necrosis factor-α (TNF-α) and chemokine ligand-10 (CXCL-10) were analyzed.ResultsAll animals in the experimental groups exhibited varying degrees of liver damage. The SPECT images and indexes (HLI5 and LHL15) of the experimental groups significantly differed from those of the control group (P P ConclusionUsing SPECT with 99mTc-EHIDA, along with the calculated indexes and levels of various cytokines, presents a dependable method for assessing liver function.

目的：采用动态肝胆单光子发射计算机断层扫描（single-photon emission computed tomography, SPECT）评估不同急性肝衰竭大鼠模型的肝功能。方法：将24只6~8周龄雄性Sprague-Dawley大鼠（体重190~200g）平均分为四组。通过腹腔注射D-半乳糖胺（D-galactosamine, D-GalN，600mg/kg）联合脂多糖（lipopolysaccharide, LPS，10μg/kg）、胆总管结扎术、切除70%肝组织三种方式构建急性肝衰竭模型，第四组作为空白对照组，不予任何干预。以99m锝-亚乙基亚氨基二乙酸（99mTc-ethylene hepatobiliary iminodiacetic acid, EHIDA）行动态SPECT扫描，获取肝脏与心脏的时间-活性曲线，计算图像衍生功能参数：5分钟心肝指数（5-minute heart/liver index, HLI5）与15分钟受体指数（15-minute receptor index, LHL15）。此外，分析图像衍生参数与血清白细胞介素-6（interleukin-6, IL-6）水平、肝脏天冬氨酸氨基转移酶（aspartate aminotransferase, AST）及丙氨酸氨基转移酶（alanine transaminase, ALT）水平，以及肝脏肿瘤坏死因子-α（tumor necrosis factor-α, TNF-α）、趋化因子配体-10（chemokine ligand-10, CXCL-10）的mRNA表达水平的相关性。结果：各实验组动物均出现不同程度的肝损伤。实验组的SPECT影像及HLI5、LHL15指标与对照组相比均存在显著差异（P P）。结论：采用99mTc-EHIDA标记的SPECT联合所计算的功能指标及各类细胞因子水平，可为肝功能评估提供可靠方法。