Genotype-by-diet interaction effects on transcriptome profiles in Drosophila melanogaster

This study was performed by testing 21 wildtype genetic lines on four different diets to look at the transcriptomic, metabolomic, and gross phenotype variation. The diets used were Normal Cornmeal-molasses symbolized N, high sugar symbolized 4, high fat F, and control C a low sugar diet. Larvae were raised from hatching to 3rd instar on the respecitve diets at a density of 50 larvae per food vial. Larvae were then collected for metabolomics, transcriptomic, and gross phenotype measurements (e.g. trehalose concentration, triglyceride concentration). Samples for transcriptomic and metabolomic analyses were snap frozen in liquid nitrogen. 236 total samples were analyzed. In most cases the are unique technical replicates except in the cases where the line, diet, and biological replicate characterists are identical.本研究针对21个野生型遗传品系，在四种不同日粮条件下开展测试，旨在探究其转录组学、代谢组学及整体表型的变异特征。本次实验所用日粮分别为：标记为N的普通玉米粉-糖蜜日粮、标记为4的高糖日粮、标记为F的高脂日粮，以及标记为C的低糖对照日粮。幼虫从孵化至3龄阶段均在对应日粮中饲养，每支饲养管的饲养密度为50头幼虫。随后收集幼虫，用于代谢组学、转录组学及整体表型检测（检测指标包括海藻糖浓度、甘油三酯浓度等）。用于转录组学和代谢组学分析的样本经液氮快速冷冻处理。本研究共分析236例样本，多数样本为独立技术重复，仅当品系、日粮及生物学重复特征完全一致时除外。