MicroRNA in plasma, Newly diagnosed untreated diabetic cases and healthy controls. MicroRNA in plasma, Newly diagnosed untreated diabetic cases and healthy controls

We examined the plasma miRNA among 113 newly diagnosed untreated diabetic cases and 113 healthy controls from DFTJ cohort by miRNA array screening and Taqman real-time PCR validation. After prediction of miRNA target genes, the low-abundance proteins coded by miR-193b-3p target genes were explored in plasma of the same population. We further validated the target effect and potential cell function of differentially expressed miRNA in HepG2 cells. Overall design: we examined the plasma miRNA among 113 newly diagnosed untreated diabetic cases and 113 healthy controls from DFTJ cohort

本研究依托DFTJ队列，纳入113例新诊断未治疗的糖尿病患者与113例健康对照，通过miRNA芯片筛选（miRNA array screening）与Taqman实时荧光定量PCR（Taqman real-time PCR）验证，对受试者血浆中的微小RNA（miRNA）进行检测。在完成miRNA靶基因预测后，针对同一队列人群血浆中由miR-193b-3p靶基因编码的低丰度蛋白展开分析。本研究进一步在HepG2细胞中验证了差异表达miRNA的靶调控效应与潜在细胞功能。实验设计概述：本研究对DFTJ队列中113例新诊断未治疗糖尿病患者与113例健康对照的血浆miRNA进行了检测。