ChIP-Seq of OCT4, NANOG, p300 in 3iL Human Embryonic Stem Cells

Human embryonic stem cells (hESCs) are isolated from the inner cell mass of the blastocysts. The pluripotent properties of hESCs enable the derivation of cell-types or tissues of different lineages for potential applications such as therapeutics discovery and regenerative medicine. Even though hESCs are pluripotent, differences have been observed when compared to the native pluripotent epiblast cells of the blastocyst. We use a chemical approach (3iL: 3 small molecule inhibitor and cytokine) to induce an expression signature that more closely resembles native pluripotent cells. This experiment is the transcription factor binding data of the study.人类胚胎干细胞（human embryonic stem cells，hESCs）可从囊胚的内细胞团中分离得到。人类胚胎干细胞的多能特性使其能够分化得到不同谱系的细胞类型或组织，可用于药物发现、再生医学等潜在应用场景。尽管人类胚胎干细胞具备多能性，但与囊胚的天然多能上胚层细胞相比，已被观测到二者存在差异。本研究采用化学诱导策略（3iL：3种小分子抑制剂与细胞因子），以诱导出更接近天然多能细胞的表达特征。本实验数据即为该研究的转录因子结合数据集。