Details of the 3TC method performance for the comparisons RIN 10 vs. RIN 8.

By setting the length restriction to 1500 nt the percentage of DEGs go from 35.8% down to 3.5% with little loss of sensitivity, from 1.00 to 0.97. When the length restriction is set to 200 nt the loss of sensitivity becomes considerable. Details of the other comparisons are found in Table S2.将长度限制设置为1500 nt（核苷酸，nucleotide）时，差异表达基因（Differentially Expressed Genes，DEGs）的占比从35.8%降至3.5%，且灵敏度仅出现小幅下降，从1.00降至0.97。当长度限制设置为200 nt时，灵敏度的下降幅度则较为显著。其余对比分析的详细信息可见补充表S2（Table S2）。