PTEN loss in the fallopian tube induces hyperplasia and ovarian tumor formation. PTEN loss in the fallopian tube induces hyperplasia and ovarian tumor formation

The goals of this study are to compare mouse oviductal transcriptome profiling (RNA-seq) of PTEN-depleted cells by shRNA versus a scr shRNA control expressing PTEN. Overall design: mRNA profiles of PTEN-deficient and control scramble Mouse oviductal cells were generated by deep sequencing, in triplicate, using Illumina HiSeq2500.

本研究旨在通过RNA测序（RNA-seq），对比经短发夹RNA（shRNA）敲低PTEN的小鼠输卵管细胞与转染阴性对照短发夹RNA（scr shRNA）且表达PTEN的小鼠输卵管细胞的转录组谱。
实验整体设计：采用Illumina HiSeq2500平台对PTEN敲低型与阴性对照小鼠输卵管细胞的mRNA表达谱进行深度测序，每组设置3次生物学重复。