liver dcd project

Organ transplantation is considered the therapy of choice for end-stage organ failure. However, the growing gap between the number of patients waiting for transplantation and available organs continue to be the number one issue facing the transplant community. As a consequence the major focus in liver transplantation has been developing strategies to increase available donor. The use of organs procured from donors after circulatory death has increased the pool of potential liver donors. However, donation after cardiac Death (DCD) livers are at increased risk of primary graft dysfunction. We conducted RNA-sequencing experiments using DCD livers from the rat model to assess the short-term feasibility and functional efficacy of DCD livers. Using RNA-sequencing data, we found that liver transcriptome level would have a great change in rat livers subjected to 15 minutes of cardiac arrest, which indicated a great loss of normal function of DCD livers before transplantation.器官移植被视为终末期器官衰竭的首选治疗方案。然而，等待移植的患者数量与可获取供器官之间的缺口持续扩大，已成为移植领域面临的首要难题。因此，肝移植领域的核心研究方向已转向开发扩增可获取供体的策略。采用循环死亡后捐赠者的供器官，已扩大了潜在肝供体库。不过，心脏死亡后捐赠（Donation after Cardiac Death, DCD）的肝脏，其发生原发性移植功能障碍的风险显著升高。本研究借助大鼠模型的DCD肝脏样本开展RNA测序实验，以评估DCD肝脏的短期可行性与功能效能。通过RNA测序数据，我们发现经历15分钟心脏停搏的大鼠肝脏，其肝脏转录组水平会发生显著变化，这表明移植前DCD肝脏的正常功能已出现大幅损伤。